Liver is responsible for concentrating and metabolizing a majority of medications, so it is a prime target for medication-induced damage
(the next targets are kidney and heart).
Drug-induced liver injury (DILI) is also one of the main reasons for drug withdrawal from the market.
Detecting DILI as early as possible during drug development is crucial.
Low doses are ineffective and high doses are toxic.
Determining the toxic dose is an important aspect of this procedure.